Fig. 2. Knockdown of MALAT1 suppressed growth and induced apoptosis of Hep3B cells after hypoxia challenge. Hep3B cells were transfected with si-NC or si-MALAT1. For N-control group, cells were cultured under normoxia condition for 72 hours; for H-control group, cells were first challenged with hypoxia (1% oxygen) for 24 hours, followed by culturing under normoxia condition for 48 hours. (A) MALAT1 expression level in each treatment group was measured by qRT-PCR. (B) Hep3B cell proliferation in each treatment group was evaluated by MTT assay. (C and D) Hep3B cell apoptotic rate in each treatment group was detected by flow cytometry. ^*p<0.05: when compared with N-control group, ^†p<0.05: when compared with H-si-NC group. MALAT1, metastasis-associated lung adenocarcinoma transcript 1.