Fig. 3.
No CD4 T cell reactivity against IDH1R132H despite long-term pre-culture of CD4 T cells in presence of antigen. a Scheme of co-culture system used to enrich frequencies of mutant-specific T cells. In short, HLA-DR1 or DR4-positive patient PBMC were co-cultured with HLA-DR1 or -DR4 transduced HeLa cells loaded with IDH1R132H peptide or no peptide for 4 weeks. Prior to measurements of T cell activation, T cell populations (n = 6 patients) were stimulated with DR1 or DR4-positive HeLa cells loaded with IDH1R132H or IDH1WT peptide for 16 h. b Percentage of CD137+ cells of CD4+ T cells. c IFN-γ response of the same T cell populations used in (b). Each dot represents an individual patient sample. DR1 + R132H, DR1 + WT, DR4 + R132H, DR4 + WT were corrected for the medium values. DR1, HeLa cells transduced with DRB1*01:01. DR4, HeLa cells transduced with DRB1*04:01