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. 2019 Jun 25;144(1):79–87. doi: 10.1007/s11060-019-03228-6

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Tumors of LGG patients do not harbor CD4 T cell reactivity against IDH1^R132H. TILs were obtained as described and expanded from fresh LGG tumor fragments in IL-2 supplemented medium during 2 weeks. TILs were then stimulated with IDH1^R132H or IDH1^WT peptide-loaded, irradiated autologous PBMC for 16 h (n = 10 patients). a Percentage of CD137+ cells of CD4+ T cells. b IFN-γ response of the same TIL populations as in (a). Each dot represents an individual patient sample. IDH1^R132H and IDH1^WT were corrected for the medium values