Fig. 3.

Light microscope image of a coronal brain slice illustrating the guide and internal cannula tracks (black and blue arrows, respectively). The cannulas are considered to be in the DMH if the tips (indicated by the red arrows) are located ventral to the mammilothalamic tract (mt), medial to the fornix (f), and between 2.6 to 3.8 mm posterior to bregma (this image is from (1) and illustrates cannula tracks from an animal used in that study).