Figure 5.

MYO1B Depletion Promotes Apoptosis of Immortalized MEF Cells

(A) Immortalized MEF cells were transduced with rAd/U6-LacZ-shRNA as control and rAd/U6-MYO1B-shRNA for silencing MYO1B. After 3 days of transduction and 16 h of serum starvation (A) apoptotic cells were detected by Annexin-V-FLUOS staining (upper panel). The lower panels show phase contrast images.

(B) Plot graphs present quantification of Annexin-V-positive apoptotic cells shown in (A).

(C) Immunoblotting analysis of MYO1B, caspase 3 (CASP3), cleaved CASP3, and PCNA.

(D) Quantification of the signals in (C).

(E) Viable cell number.

Scale bar, 100 μm. All values are presented as mean ± SEM of the data from three to four independent sets of experiments; t test; *p < 0.05, **p < 0.01 versus LacZ-shRNA control. shRNA, short hairpin RNA.