Specifications table

Subject area	Proteomics
More specific subject area	DIA based mass spectrometric analysis of human tear fluid
Type of data	Raw files, msf.files
How data was acquired	Mass spectrometry (Q Exactive HF mass spectrometer operated in data-dependent acquisition (DDA) mode performing HCD fragmentation)
Data format	Raw files, unfiltered
Experimental factors	Data were obtained by mass spectrometric DDA measurements of human tear fluid spiked with iRT peptides for use as a spectral library.
Experimental features	Tear fluid was fractionated via gel electrophoresis and in-gel digested resulting in 48 bands.
Data source location	Bochum, Germany (51°26′43.4″N 7°15′27.9″E)
Data accessibility	The data files are hosted in the public repository ProteomeXchange with identifier PXD011075.
Related research article	Barkovits K., Linden A., Galozzi S., Schilde L., Pacharra S., Mollenhauer B., Stoepel N., Steinbach S., May C., Uszkoreit J., Eisenacher M., Marcus K.: Characterization of Cerebrospinal Fluid via Data-Independent Acquisition Mass Spectrometry[1] Published in J Proteome Res. 2018