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. 2019 Jul 22;12:181–194. doi: 10.2147/JIR.S213594

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© 2019 Eliasse et al.

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DC_ASW exhibit a reduced ability to induce CD4⁺ T cell proliferation. CTV-loaded allogeneic naive CD4⁺ T cells were cocultured with indicated DCs at T cell:DC ratio of 1:10, 1:20 or 1:40 for 5 days and CTV dilution was analyzed by flow cytometry in CD4⁺ T cells. (A) Representative histograms; numbers indicate the percentage of proliferating CD4⁺ T cells. (B) Pooled data from n=27 donors, box and whiskers representation (minimum, first quartile, median, third quartile, and maximum). **p<0.01, ***p<0.001, ****p<0.0001, One-way ANOVA (Dunnett’s multiple comparison test).

Abbreviations: ASW, Avène thermal spring water; DC, dendritic cell; CTW, control water; DEX, dexamethasone; CTV, Celltrace violet.