Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 26;19:169. doi: 10.1186/s12866-019-1544-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 2 — YpeB_∆25–203-Cys can be cross-linked in spores. Ten optical density units of decoated dormant spores were cross-linked with APB as described previously. Cross-linked spores were lyophilized and mechanically broken, and proteins were extracted. Whole spore lysates were then visualized via western blot using anti-YpeB antibodies [20]. The positions of protein size markers (lane 1) are indicated on the left. YpeB monomer is indicated by the arrow at 50 kDa. YpeB_∆25–203 monomer is indicated at roughly ~ 30 kDa. YpeB multimers are visualized in YpeB^K437C (lane 6) migrating at 100 and 150 kDa. YpeB_∆25–203 multimers are indicated in YpeB_∆25-203K437C (lane 8) migrating ~ 60 kDa. A putative YpeB-YpeB_∆25–203 heterodimer (lane 10) is indicated at ~ 80 kDa, in addition to homodimers identified in previous lanes