Table 1.
Bacterial strains and plasmids
| Strain | Genotype | Constructiona | Source |
|---|---|---|---|
| B. anthracis | |||
| Sterne 34F2 | pXO1+ pXO2− | P. Hanna | |
| DPBa89 | ∆ypeB | pDPV392 > 34F2 | [20] |
| DPBa127 | ∆ypeB::pDPV424 (YpeB1–446-His6 ErR) | pDPV424 > DPBa89 | [20] |
| DPBa204 | ∆ypeB::pDPV476 (YpeBK437C-His6 ErR) | pDPV476 > DPBa89 | This study |
| DPBa205 | ∆ypeB::pDPV477 (YpeBS358C-His6 ErR) | pDPV477 > DPBa89 | This study |
| DPBa210 | ∆ypeB::pDPV478 (YpeBT328C-His6 ErR) | pDPV478 > DPBa89 | This study |
| DPBa211 | ∆ypeB::pDPV479 (YpeBM282C-His6 ErR) | pDPV479 > DPBa89 | This study |
| DPBa212 | ∆ypeB::pDPV480 (YpeBY339C-His6 ErR) | pDPV480 > DPBa89 | This study |
| DPBa213 | ∆ypeB::pDPV481 (YpeBE314C-His6 ErR) | pDPV481 > DPBa89 | This study |
| DPBa214 | ∆ypeB::pDPV482 (YpeBV355C-His6 ErR) | pDPV482 > DPBa89 | This study |
| DPBa215 | ∆ypeB::pDPV483 (YpeBV324C-His6 ErR) | pDPV483 > DPBa89 | This study |
| DPBa216 | ∆ypeB::pDPV484 (YpeBT420C-His6 ErR) | pDPV484 > DPBa89 | This study |
| DPBa217 | ∆ypeB::pDPV485 (YpeBA342C-His6 ErR) | pDPV485 > DPBa89 | This study |
| DPBa218 | ∆ypeB::pDPV486 (YpeBV435C-His6 ErR) | pDPV486 > DPBa89 | This study |
| DPBa219 | ∆ypeB::pDPV487 (YpeBQ438C-His6 ErR) | pDPV487 > DPBa89 | This study |
| DPBa220 | ∆ypeB::pDPV488 (YpeB∆25–203 K437C-His6 ErR) | pDPV488 > DPBa89 | This study |
| DPBa221 | pDPV488 (YpeB∆25–203 K437C-His6 ErR) | pDPV488 > 34F2 | This study |
| DPBa222 | ∆ypeB::pDPV489 (YpeB∆25–203 S358C-His6 ErR) | pDPV489 > DPBa89 | This study |
| DPBa223 | pDPV489 (YpeB∆25–203 S358C-His6 ErR) | pDPV489 > 34F2 | This study |
| E.coli | |||
| BTH101 | Euromedex | ||
| DPVE545 | pKT25-zip + pUT18C-zip | pKT25-zip + pUT18C-zip > BTH101 | Euromedex |
| DPVE546 | pKT25 + pUT18C | This study | |
| DPVE547 | p25-YpeB21–446 + p18-YpeB21–446 | pDPV494 + pDPV490 > BTH101 | This study |
| DPVE548 | p25-YpeB21–446 + p18-YpeB21–202 | pDPV494 + pDPV491 > BTH101 | This study |
| DPVE549 | p25-YpeB21–446 + p18-YpeB203–446 | pDPV494 + pDPV492 > BTH101 | This study |
| DPVE550 | p25-YpeB21–446 + p18-SleB125–253 | pDPV494 + pDPV493 > BTH101 | This study |
| DPVE551 | p25-YpeB21–202 + p18-YpeB21–446 | pDPV495 + pDPV490 > BTH101 | This study |
| DPVE552 | p25-YpeB21–202 + p18-YpeB21–202 | pDPV495 + pDPV491 > BTH101 | This study |
| DPVE553 | p25-YpeB21–202 + p18-YpeB203–446 | pDPV495 + pDPV492 > BTH101 | This study |
| DPVE554 | p25-YpeB21–202 + p18-SleB125–253 | pDPV495 + pDPV493 > BTH101 | This study |
| DPVE555 | p25-YpeB203–446 + p18-YpeB21–446 | pDPV496 + pDPV490 > BTH101 | This study |
| DPVE556 | p25-YpeB203–446 + p18-YpeB21–202 | pDPV496 + pDPV491 > BTH101 | This study |
| DPVE557 | p25-YpeB203–446 + p18-YpeB203–446 | pDPV496 + pDPV492 > BTH101 | This study |
| DPVE558 | p25-YpeB203–446 + p18-SleB125–253 | pDPV496 + pDPV493 > BTH101 | This study |
| DPVE559 | p25-SleB125–253 + p18-YpeB21–446 | pDPV497 + pDPV490 > BTH101 | This study |
| DPVE560 | p25-SleB125–253 + p18-YpeB21–202 | pDPV497 + pDPV491 > BTH101 | This study |
| DPVE561 | p25-SleB125–253 + p18-YpeB203–446 | pDPV497 + pDPV492 > BTH101 | This study |
| DPVE562 | p25-SleB125–253 + p18-SleB125–253 | pDPV497 + pDPV493 > BTH101 | This study |
| Plasmids | |||
| pBKJ236 | [27] | ||
| pKT25-zip | Euromedex | ||
| pUT18C-zip | Euromedex | ||
| pKT25 | Euromedex | ||
| pUT18C | Euromedex | ||
| pDPV392 | pBKJ236::∆ypeB | [20] | |
| pDPV424 | pBKJ236::∆sleB ypeB1–446-His6 | [20] | |
| pDPV448 | pBKJ236::∆sleB ypeB∆25–203-His6 | [20] | |
| pDPV476 | pBKJ236::∆sleB ypeBK437C-His6 | This study | |
| pDPV477 | pBKJ236::∆sleB ypeBS358C-His6 | This study | |
| pDPV478 | pBKJ236::∆sleB ypeBT328C-His6 | This study | |
| pDPV479 | pBKJ236::∆sleB ypeBM282C-His6 | This study | |
| pDPV480 | pBKJ236::∆sleB ypeBY339C-His6 | This study | |
| pDPV481 | pBKJ236::∆sleB ypeBE314C-His6 | This study | |
| pDPV482 | pBKJ236::∆sleB ypeBV355C-His6 | This study | |
| pDPV483 | pBKJ236::∆sleB ypeBV324C-His6 | This study | |
| pDPV484 | pBKJ236::∆sleB ypeBT420C-His6 | This study | |
| pDPV485 | pBKJ236::∆sleB ypeBA324C-His6 | This study | |
| pDPV486 | pBKJ236::∆sleB ypeBV435C-His6 | This study | |
| pDPV487 | pBKJ236::∆sleB ypeBQ438C-His6 | This study | |
| pDPV488 | pBKJ236::∆sleB ypeB∆25–203 K437C-His6 | This study | |
| pDPV489 | pBKJ236::∆sleB ypeB∆25–203 S358C-His6 | This study | |
| pDPV490 | pUT18C::ypeB21–446 | This study | |
| pDPV491 | pUT18C::ypeB21–202 | This study | |
| pDPV492 | pUT18C::ypeB203–446 | This study | |
| pDPV493 | pUT18C::sleB125–253 | This study | |
| pDPV494 | pKT25::ypeB21–446 | This study | |
| pDPV495 | pKT25::ypeB21–202 | This study | |
| pDPV496 | pKT25::ypeB203–446 | This study | |
| pDPV497 | pKT25::sleB125–253 | This study | |
a > indicates transformation of the indicated plasmid into the indicated strain