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. 2019 Jul 26;19:169. doi: 10.1186/s12866-019-1544-1

Table 1.

Bacterial strains and plasmids

Strain Genotype Constructiona Source
B. anthracis
 Sterne 34F2 pXO1+ pXO2 P. Hanna
 DPBa89 ypeB pDPV392 > 34F2 [20]
 DPBa127 ypeB::pDPV424 (YpeB1–446-His6 ErR) pDPV424 > DPBa89 [20]
 DPBa204 ypeB::pDPV476 (YpeBK437C-His6 ErR) pDPV476 > DPBa89 This study
 DPBa205 ypeB::pDPV477 (YpeBS358C-His6 ErR) pDPV477 > DPBa89 This study
 DPBa210 ypeB::pDPV478 (YpeBT328C-His6 ErR) pDPV478 > DPBa89 This study
 DPBa211 ypeB::pDPV479 (YpeBM282C-His6 ErR) pDPV479 > DPBa89 This study
 DPBa212 ypeB::pDPV480 (YpeBY339C-His6 ErR) pDPV480 > DPBa89 This study
 DPBa213 ypeB::pDPV481 (YpeBE314C-His6 ErR) pDPV481 > DPBa89 This study
 DPBa214 ypeB::pDPV482 (YpeBV355C-His6 ErR) pDPV482 > DPBa89 This study
 DPBa215 ypeB::pDPV483 (YpeBV324C-His6 ErR) pDPV483 > DPBa89 This study
 DPBa216 ypeB::pDPV484 (YpeBT420C-His6 ErR) pDPV484 > DPBa89 This study
 DPBa217 ypeB::pDPV485 (YpeBA342C-His6 ErR) pDPV485 > DPBa89 This study
 DPBa218 ypeB::pDPV486 (YpeBV435C-His6 ErR) pDPV486 > DPBa89 This study
 DPBa219 ypeB::pDPV487 (YpeBQ438C-His6 ErR) pDPV487 > DPBa89 This study
 DPBa220 ypeB::pDPV488 (YpeB∆25–203 K437C-His6 ErR) pDPV488 > DPBa89 This study
 DPBa221 pDPV488 (YpeB∆25–203 K437C-His6 ErR) pDPV488 > 34F2 This study
 DPBa222 ypeB::pDPV489 (YpeB∆25–203 S358C-His6 ErR) pDPV489 > DPBa89 This study
 DPBa223 pDPV489 (YpeB∆25–203 S358C-His6 ErR) pDPV489 > 34F2 This study
E.coli
 BTH101 Euromedex
 DPVE545 pKT25-zip + pUT18C-zip pKT25-zip + pUT18C-zip > BTH101 Euromedex
 DPVE546 pKT25 + pUT18C This study
 DPVE547 p25-YpeB21–446 + p18-YpeB21–446 pDPV494 + pDPV490 > BTH101 This study
 DPVE548 p25-YpeB21–446 + p18-YpeB21–202 pDPV494 + pDPV491 > BTH101 This study
 DPVE549 p25-YpeB21–446 + p18-YpeB203–446 pDPV494 + pDPV492 > BTH101 This study
 DPVE550 p25-YpeB21–446 + p18-SleB125–253 pDPV494 + pDPV493 > BTH101 This study
 DPVE551 p25-YpeB21–202 + p18-YpeB21–446 pDPV495 + pDPV490 > BTH101 This study
 DPVE552 p25-YpeB21–202 + p18-YpeB21–202 pDPV495 + pDPV491 > BTH101 This study
 DPVE553 p25-YpeB21–202 + p18-YpeB203–446 pDPV495 + pDPV492 > BTH101 This study
 DPVE554 p25-YpeB21–202 + p18-SleB125–253 pDPV495 + pDPV493 > BTH101 This study
 DPVE555 p25-YpeB203–446 + p18-YpeB21–446 pDPV496 + pDPV490 > BTH101 This study
 DPVE556 p25-YpeB203–446 + p18-YpeB21–202 pDPV496 + pDPV491 > BTH101 This study
 DPVE557 p25-YpeB203–446 + p18-YpeB203–446 pDPV496 + pDPV492 > BTH101 This study
 DPVE558 p25-YpeB203–446 + p18-SleB125–253 pDPV496 + pDPV493 > BTH101 This study
 DPVE559 p25-SleB125–253 + p18-YpeB21–446 pDPV497 + pDPV490 > BTH101 This study
 DPVE560 p25-SleB125–253 + p18-YpeB21–202 pDPV497 + pDPV491 > BTH101 This study
 DPVE561 p25-SleB125–253 + p18-YpeB203–446 pDPV497 + pDPV492 > BTH101 This study
 DPVE562 p25-SleB125–253 + p18-SleB125–253 pDPV497 + pDPV493 > BTH101 This study
Plasmids
 pBKJ236 [27]
 pKT25-zip Euromedex
 pUT18C-zip Euromedex
 pKT25 Euromedex
 pUT18C Euromedex
 pDPV392 pBKJ236::∆ypeB [20]
 pDPV424 pBKJ236::∆sleB ypeB1–446-His6 [20]
 pDPV448 pBKJ236::∆sleB ypeB25–203-His6 [20]
 pDPV476 pBKJ236::∆sleB ypeBK437C-His6 This study
 pDPV477 pBKJ236::∆sleB ypeBS358C-His6 This study
 pDPV478 pBKJ236::∆sleB ypeBT328C-His6 This study
 pDPV479 pBKJ236::∆sleB ypeBM282C-His6 This study
 pDPV480 pBKJ236::∆sleB ypeBY339C-His6 This study
 pDPV481 pBKJ236::∆sleB ypeBE314C-His6 This study
 pDPV482 pBKJ236::∆sleB ypeBV355C-His6 This study
 pDPV483 pBKJ236::∆sleB ypeBV324C-His6 This study
 pDPV484 pBKJ236::∆sleB ypeBT420C-His6 This study
 pDPV485 pBKJ236::∆sleB ypeBA324C-His6 This study
 pDPV486 pBKJ236::∆sleB ypeBV435C-His6 This study
 pDPV487 pBKJ236::∆sleB ypeBQ438C-His6 This study
 pDPV488 pBKJ236::∆sleB ypeB∆25–203 K437C-His6 This study
 pDPV489 pBKJ236::∆sleB ypeB∆25–203 S358C-His6 This study
 pDPV490 pUT18C::ypeB21–446 This study
 pDPV491 pUT18C::ypeB21–202 This study
 pDPV492 pUT18C::ypeB203–446 This study
 pDPV493 pUT18C::sleB125–253 This study
 pDPV494 pKT25::ypeB21–446 This study
 pDPV495 pKT25::ypeB21–202 This study
 pDPV496 pKT25::ypeB203–446 This study
 pDPV497 pKT25::sleB125–253 This study

a > indicates transformation of the indicated plasmid into the indicated strain