Fig. 1.

AZD7762 enhance the effect of cisplatin on human osteosarcoma cells. a, b MTS assay analyzes the inhibitory effect of AZD7762 and cisplatin on the proliferation of osteosarcoma cell lines. HOS and Saos-2 cell were treated with different concentration of AZD7762 and cisplatin for 24 h, respectively. The results are the mean of six replicates, and each experiment performed in triplicate. c, d The inhibitory effect detected by MTS assay that AZD77762 (100 nmol/L) in combination with different concentration of cisplatin on HOS and Saos-2 cells for 24 h. The results are the mean of six replicates, and each experiment performed in triplicate. e Human osteosarcoma cells HOS and Saos-2 were treated with PBS (control group), cisplatin (10 μmol/L), AZD7762 (100 nmol/L) and cisplatin + AZD7762 for 24 h. The expression level of apoptosis-related protein PARP, cleaved caspase-3, -9 and Bax were determined by western blot. *p < 0.05 and **p < 0.01 versus control group. ^#p < 0.05 and ^##p < 0.01 versus cisplatin treatment with the same concentration. FL full length, CL cleaved