Fig. 2.

Analysis of AZD7762 in combination with Cisplatin on cell cycle of osteosarcoma cells. a, b HOS and Saos-2 cells were treated with PBS (control group), cisplatin (10 μmol/L), AZD7762 (100 nmol/L) and cisplatin + AZD7762 for 24 h. The data of cell cycle distribution detected by flow cytometry were obtained from three independent experiments. *p < 0.05 and **p < 0.01 versus control group. c Human osteosarcoma cells HOS and Saos-2 were treated with cisplatin (10 μmol/L) alone or in combination with AZD7762 (100 nmol/L) for 24 h. The expression of cell cycle related protein of p-Chk1, p-Cdc25C, p-Cdc2, Cyclin B1 and phosphorylated histone H2A.X were measured by western blot. ^#p < 0.05 and ^##p < 0.01 versus cisplatin treatment with the same concentration