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Capillary chemotaxis assays with Td confirm a role for ODP in sensing oxygen and iron. (A) Oxygenated buffer is a chemorepellent for Td WT but not ∆2498 (-ODP) cells. Only WT cells show less migration into capillaries when the buffer is oxygenated compared with anaerobic controls. (B) WT cells showed strong attraction to ferrous iron, but the ∆2498 cell line did not. (C) Both the WT and ∆2498 recognized glucose as a chemoattractant.
