Table 2.
Kinetic parameters of LRRK2 constructs
| LRRK2 construct | KM(ATP), µM | kcat, 1/min | kcat/KM, 1/(min µM) |
| wt | 136 ± 23 | 140 ± 32 | 1.1 ± 0.3 |
| A1904F (CS8) | — | Inactive | — |
| L1924F (RS3) | 113 ± 18 | 40 ± 6 | 0.36 ± 0.06 |
| L1935F (RS4) | 76 ± 22 | 14 ± 12 | 0.10 ± 0.04 |
| Y1992F (RS1) | 204 ± 25 | 45 ± 9 | 0.23 ± 0.06 |
| D2017A (DFGψ) | — | Inactive | — |
| Y2018F (RS2/DYGψ) | 85 ± 12 | 413 ± 40 | 4.9 ± 0.9 |
| G2019S (DFGψ) | 138 ± 15 | 322 ± 35 | 2.4 ± 0.2 |
| I2020T (DFGψ) | 43 ± 18 | 79 ± 12 | 2.3 ± 1.5 |
| F1908L (HN1) | 85 ± 9 | 17.4 ± 0.8 | 0.21 ± 0.02 |
Values are given as mean ± SD from at least 2 independent protein preparations with each preparation measured at least twice except for F1908L, where 1 protein preparation was measured in 4 independent experiments.