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Multichannel 3D imaging enables detailed imaging of micrometastases. Light-sheet imaging was used to capture individual channels for nuclei (DAPI), cancer cells (Ki67), blood vessels (GSL-1), and nanoparticles (darkfield) that can be viewed individually or as a composite image. An example of an individual liver micrometastasis is shown as 2D slices from a 3D image. (Scale bar, 50 μm.) NP, nanoparticles.
