Fig. 2.

The increased population of Iba-1+ cells in the inner retina after BRVO is partly due to blood-derived macrophage infiltration. Representative images of the inner aspect of Iba1 (green), ColIV (white), and EdU (red) triple-labeled retina of control animals (a) and 3 days after BRVO (b) that were intraperitoneally injected with EdU for 4 days. The insets depict representative EdU+Iba1+ parenchymal (left panel) and perivascular (right panel) Mφs for each condition at higher magnification as they are not visible in the overview. Quantification of Iba1⁺ Mφs (white columns) and Iba1⁺EdU⁺Mφs (black columns) of the parenchymal Mφs per square millimeter (c, n = 6/group, Mann-Whitney t test, control parenchymal Iba1⁺EdU⁺Mφs versus BRVO liposome parenchymal Iba1⁺EdU⁺Mφs *P = 0.0143; BRVO liposome parenchymal Iba1⁺EdU⁺Mφs versus BRVO liposome clodronate parenchymal Iba1⁺EdU⁺Mφs ^$P = 0.0143) and the number of perivascular Iba1⁺ Mφs per millimeter of vein (d, n = 6/group, Mann-Whitney t test, control perivascular Iba1⁺EdU⁺Mφs versus BRVO liposome parenchymal Iba1⁺EdU⁺Mφs *P = 0.0143; BRVO liposome parenchymal Iba1⁺EdU⁺Mφs versus BRVO liposome clodronate perivascular Iba1⁺EdU⁺Mφs ^$P = 0.0095) of control retinas and retinas from BRVO mice receiving intravenous control liposome injections (lipo) or clodronate liposome injections (lipo-clo). BRVO, branch retinal vein occlusion; lipo, control liposome-treated; lipo-clo, liposome clodronate-treated; Iba1, ionized calcium-binding adapter molecule 1. Scale bar a and b = 10 μm. Inset = 5 μm