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Cas9-TV and proximal dsgRNA alter local chromatin accessibility. Rice protoplasts were transfected with Cas9, Cas9-TV, Cas9/dsgRNA, and Cas9-TV/dsgRNA, respectively, and local chromatin accessibility around target sites was analyzed by low-input DNase I assays. The fractions of intact genomic DNA were quantified by real-time PCR. For each site, relative amount of intact genomic DNA in the Cas9-treated sample was set as one unit. Error bars indicate SD of three replicates
