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. 2019 Jul 26;20:145. doi: 10.1186/s13059-019-1762-8

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Comparison of off-target activities of Cas9, Cas9-TV, and Cas9-TV/dsgRNA. Indel frequencies were measured by sequencing targeted amplicons in rice protoplasts. An untreated protoplast sample was used as a control. Data are from sets of three independent biological replicates (n = 3) and are shown as means ± s.e.m