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Combined treatment of bicarbonate and different chemotherapeutic agents: (A+B) Cisplatin (pKa 5.06, 3.8μg/ml) and 5-fluorouracil (5FU, pKa 8.02, 1.3μg/ml) effect on 4T1 breast cancer cells after combination with bicarbonate (50mM) and HEPES buffer (50mM). (C) Summary of bicarbonate effect on chemotherapeutic agents' activity on 4T1 cells after a combined treatment.
