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. Author manuscript; available in PMC: 2019 Jul 28.
Published in final edited form as: Cell Rep. 2019 Jul 2;28(1):39–50.e4. doi: 10.1016/j.celrep.2019.05.111

Figure 5. The N-Terminal Half of Hcr1 Is Required for Its Function in Ribosome Recycling.

Figure 5.

(A) Schematic of the Hcr1 protein showing the lengths (aa) of the HCR1-NTD and HCR1-CTD gene fragments (left), and the ribosome footprint profile of HCR1 confirming expression of HCR1-NTD and HCR1-CTD in the hcr1Δ strain (right).

(B) Normalized average ribosome footprint occupancy (equally weighted by reads within the ORF) for all genes aligned at their stop codons. Footprints plotted by 5′ ends. (Inset) Magnified view of (B) showing complementation of 3′ UTR ribosome occupancy in the hcr1Δ strain by HCR1-NTD but not HCR1-CTD.

(C) Individual examples of the elimination of 3′ UTR reads shown in the average in (B). Ribosome footprint profiles of the 3′ UTRs of genes YDR524C-B and YMR122W-A showing a loss of 3′ UTR footprint density in the hcr1Δ hcHCR1-NTD strain relative to the hcr1Δ YEplac195.