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. Author manuscript; available in PMC: 2019 Jul 28.
Published in final edited form as: Cell Rep. 2019 Jul 2;28(1):39–50.e4. doi: 10.1016/j.celrep.2019.05.111

Figure 7. RLI1 Expression Is Increased in the hcr1 Deletion Strain.

Figure 7.

(A) Analysis of Gene Ontology (GO) terms in the dataset of genes that significantly change expression (assayed by mRNA-seq) in the hcr1Δ strain. GO terms that are most significantly enriched above the expected background level are shown.

(B) Correlation analysis of fold changes in mRNA-seq between hcr1Δ and rli1-d strains (top, Spearman’s R2 = 0.27) or between hcr1Δ strains in WT and ski2Δ backgrounds (bottom, Spearman’s R2 = 0.45) to assess the effect of inhibition of nonstop decay. Genes involved in iron sulfur (Fe-S) maturation that are significantly upregulated are identified in red (LTO1, ISU2, DRE2, and CFD1).

(C) (Left) Model for Hcr1 function in the cell. (Right) Schematic diagram showing that impairment of ribosome recycling results in increased expression of RLI1 either through increased transcription (1) or a decrease in decay of the RLI1 mRNA (2).

See also Table S2.