KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal antibody c-Myc (9E10)	Roche	1167203001
Mouse monoclonal antibody ANTI-FLAG® M2	Sigma	F1804
Mouse monoclonal antibody Anti-beta actin	Abcam	ab8224
Bacterial and Virus Strains
DH5alpha	NEB	C2987H
Biological Samples
N/A	N/A	N/A
Chemicals, Peptides, and Recombinant Proteins
Cycloheximide	Sigma	C7698
RNase I	Ambion	AM2294
PNK	NEB	M0201L
Universal miRNA cloning linker	NEB	S1315S
T4 RNA ligase 2, truncated	NEB	M0242L
Superscript III Reverse Transcriptase	Invitrogen	18080044
Dynabeads MyOne streptavidin C1	Invitrogen	65001
Phusion DNA polymerase	ThermoFisher Scientific	F530L
T4 RNA ligase 2, truncated K227Q	NEB	M0351L
5′ deadenylase	NEB	M0331S
RecJ exonuclease	Biosearch Technologies	RJ411250
Critical Commercial Assays
Q5 Site-directed mutagenesis kit	NEB	E0554S
CircLigase ssDNA ligase kit	Biosearch Technologies	CL4115K
Agilent high sensitivity DNA kit	Agilent	5067-4626
5′ DNA adenylation kit	NEB	E2610L
Oligo clean & concentrator kit	Zymo Research	D4060
Deposited Data
Raw and analyzed data	This paper	GEO: GSE124204
Experimental Models: Cell Lines
N/A	N/A	N/A
Experimental Models: Organisms/Strains
A full list of yeast strains used in this study is provided in Table S4.	This paper	N/A
Oligonucleotides
A full list of oligonucleotides used in this study is provided in Tables S5 and S8.	This paper	N/A
Recombinant DNA
A full list of plasmids used in this study are provided in Tables S6.	This paper	N/A
Software and Algorithms
Igor Pro Version 7.00	Wavemetrics	15-500
Position average and metagene and makegenelist	Young et al., 2018	makeposavg makeavggene makegenelist
BOWTIE	Github	Langmead et al., 2009
DESEQ2	Bioconductor	Love et al., 2014
PANTHER 13.1	http://www.pantherdb.org/	Thomas et al., 2003
RStudio 1.1.456	RStudio	1.1.456