FIG. 1.

Liver regeneration after 2/3 partial hepatectomy is enhanced in NCoR1^Δhep mice. (A) Protein and (B) mRNA expression change of NCoR1 in the process of liver regeneration were detected by western blot and qPCR, Data are presented as mean ± SEM (n=4). *P<0.05; **P<0.01. (C) Liver weight to body weight ratio analysis in NCoR1^fl/fl and NCoR1^Δhep mice over a time course from 0 to 168 hours after 2/3 PH. Data are presented as mean ± SEM (n=5). *P<0.05; **P<0.01, NCoR1^Δhep mice compared to NCoR1^fl/fl mice at the indicated times. (D) Morphological changes of livers of NCoR1^fl/fl mice and NCoR1^Δhep mice at the 96 hours after 2/3 PH as determined by H&E staining. scale bar: 50 μm. (E) Immunohistochemical analysis of Ki67 in paraffin tissues from livers of NCoR1^fl/fl and NCoR1^Δhep mice at the indicated times after 2/3 PH (Left). Quantification of the percentage of Ki67 labeled nuclei (Right). Data are presented as mean ± SEM (n=4). The different degrees of significance were indicated as follows in the graphs: *P<0.05, **P<0.01, NCoR1^Δhep mice compared to NCoR1^fl/fl at indicated time. (F) Western blot analysis of CyclinD1, CyclinA2, CyclinB1, PCNA using RIPA extracts of NCoR1^fl/fl and NCoR1^Δhep livers obtained at the indicated times after 2/3 PH. (Two Way ANOVA plus Student’s t test for B, C; Student’s t test for E).