Skip to main content
. Author manuscript; available in PMC: 2020 Apr 21.
Published in final edited form as: Cell Rep. 2019 May 21;27(8):2370–2384.e6. doi: 10.1016/j.celrep.2019.04.086

Figure 4. CVB4 Infection Leads to Increased Insulitis and Pancreatic Fibrosis in βHIF-1α Mice.

Figure 4.

(A) Insulin and H&E staining of pancreatic sections of uninfected and CVB4-infected FC and βHIF-1α mice at 4, 7, and 21 dpi, showing insulitis (yellow arrows) and fat accumulation. Scale bar, 200 μm.

(B) Number of immune cells in insulitic area in FC (black bars) and βHIF-1α (white bars) mice at 4, 7, and 21 dpi (n = 6 per group). *p < 0.05 and **p < 0.01 versus FC mice.

(C) Immune cells in islet infiltrates in FC (black bars) and βHIF-1α (white bars) mice at 4, 7, and 21 dpi. n = 6 per group. *p < 0.05, **p < 0.01 versus FC mice.

(D) Pancreatic fat area (% of total area) in FC (black bars) and βHIF-1α (white bars) mice at 21 dpi (n = 6 per group). **p < 0.01 versus FC mice.

(E) Collagen area per pancreas (%) in FC (black bars) and βHIF-1α (white bars) mice at 21 dpi (n = 6 per group). ***p < 0.001 versus FC mice.

(F) Representative photomicrographs of Sirius Red staining from FC and βHIF-1α mice at 21 dpi, showing the degree of pancreatic fibrosis. Scale bar, 500 μm.

(G) FACS analysis: proportions of total lymphocytes, CD3+ T cells, CD4+ T helper6 cells, CD8+ cytotoxic T cells, granzyme B, CD19+ B cells, and macrophages at 7 dpi. Data are representative of 3 independent experiments, with n = 6 mice per group in each experiment. #Comparisons with uninfected and infected FC and βHIF-1α mice; *comparisons between infected FC and βHIF-1α mice.

Data are means ± SEMs.