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. Author manuscript; available in PMC: 2020 Apr 21.
Published in final edited form as: Cell Rep. 2019 May 21;27(8):2370–2384.e6. doi: 10.1016/j.celrep.2019.04.086

Figure 6. Gene Expression Changes in Human Islets after HIF-1α Knockdown and CVB4 Infection.

Figure 6.

(A) Bright-field microscopic images ofisolated human control islets in culture ± HIF-1α knockdown and ± CVB4 infection at 24 h. Magnification x20.

(B) Heatmap of gene expression changes (n = 4 individual human donors, triplicate samples from each donor per time point). *p < 0.05 and **p < 0.01 for increase versus media. ††p < 0.01 for decrease versus media control.

(C) Expression of enterovirus RNA in human control islets from 4 distinct donors, after HIF-1α knockdown and CVB4 infection collected at 24 h (n = 4 individual human donors, triplicate samples from each donor per time point). Enterovirus RNA was not detected in uninfected islets. ****p < 0.0001 versus islets infected with CVB4 alone by 2-way ANOVA with Dunnett’s correction.

Data are means ± SEMs.