Fig. 4.

Effects of HC diet on hepatic mRNA and protein expression of sulfatide-metabolizing enzymes. a-d Effect of HC diet on hepatic Cst, Arsa, Cgt and Galc mRNA expression in WT (+/+) and Ppara-null (−/−) mice. e-i Effect of HC diet on CST, ARSA, CGT, and GALC protein expression in WT (+/+) and Ppara-null (−/−) mice. Black bars indicate control groups (Con) and white bars indicate HC diet-fed groups (Cho). Values are mean ± SD (n = 6 for each group). Data were compared between two groups using two-way ANOVA. Significant differences are indicated by *p < 0.05 and **p < 0.01