Table 1.

Real-Time PCR primers for genotyping and mutants

S.N.	Primer	Nucleotide sequence (5' to 3')	Position (nt)	Polarity
Pre-S gene
1	PS1	GGGTCACCTTATTCTTGGGA	2814–2833	F
2	PS2	CCCCGCCTGTAACACGAGCA	208–189	R
3	PS3	TTGGGAACAAGATCTACAGC	2828–2847	F
4	PS4	GTCCTGATGCGATGTTCTCC	176–157	R
5	PS-B1	ATTCAAAGCCAACTCAGAAA	2946–2965	F
6	PS-B2	ACAGTATTCTGAGCAGGGCTC	105–85	R
Pre-core/core region
1	PC1	ACATAAGAGGACTCTTGGAC	1652–1671	F
2	PC2	GAAGGAAAGAAGTCAGAAGGC	1977–1957	R
3	PC3	TACTTCAAAGACTGTGTGTTTA	1704–1725	F
4	PC4	GTCAGAAGGCAAAAAAGAGA	1966–1947	R

F, forward; R, reverse