Fig. 1.

Expression of lncUSMycN in breast cancer and receive operating characteristic (ROC) curve analysis for prediction of breast cancer prognosis. (A) Amplified lncUSMycN and β-actin on 2% agarose gel; (B) confirmation of lncUSMycN expression by Sanger sequencing; (C) qRT-PCR analysis of lncUSMycN expression level in cancerous and adjacent non-cancerous breast tissues. lncUSMycN was up-regulated in tumor tissues compared to adjacent normal tissues; (D) ROC curve for lncUSMycN to discriminate between tumor and normal tissues. The area under the ROC curve (AUC) was 0.70 (p < 0.001). The sensitivity and specificity were 0.85 and 0.55, respectively, and cut-off value was 17.5 (^**p < 0.01)