Fig. 4.

Flow cytometric evaluation of apoptosis in DU145 (A and C) versus PC-3 (B and D) cells using Annexin V‐FITC/PI V and propidium iodide double staining. After 48 hours of treatment, PARP inhibition caused by AZD2461 resulted in a substantial increase in both the early and late apoptotic phase of two cell lines, in a concentration-dependent manner. Treatment by 10, 20, and 40 µM of AZD2461 caused marked apoptosis in both Pca cell lines, but the apoptotic rate was higher in PC-3 cells while raising the possibility of necrosis induction and treating both cells with higher concentrations of AZD2461. The data were expressed as mean ± SD, three independent experiments. Error bars present standard deviation. ^*p < 0.05 compared with the control