Skip to main content
. Author manuscript; available in PMC: 2020 Jun 21.
Published in final edited form as: ACS Chem Biol. 2019 Jun 7;14(6):1068–1076. doi: 10.1021/acschembio.9b00202

Figure 2.

Figure 2.

Application of ontology in describing the effects of G33I mutation and NPO thio substitutions of the substrate in the twister ribozyme (Twr). Summarized in the center are key interactions (1–4) that are highlighted in each variant/substrate by ovals in colors corresponding to their associated catalytic strategies, γ (red) and β (green). The wild-type (WT) Twr is shown in the top left, G33I mutant (top right), S(RP) substrate (bottom left), and G33I/S(RP) (bottom right). Arrows indicate transformations between the different variant/substrates. Relative kinetic rates and catalytic effects resulting from each transformation are indicated with their associated arrows. Lack of color highlighting indicates an absence (or significant weakening) of an interaction present in the wild-type and has a normal catalytic effect, whereas emphasis of color/size of highlighting indicates an inverse effect (e.g., a larger red sphere at G33:N1 (1) indicates a favorable down-shifted pKa).