Extended Data Figure 1. Patients with predicted FOXA1 mutant status have worse outcomes.

(a) Co-crystal structure of the FKHD domain of FOXA3 in complex with DNA resembling the FKHD consensus sequence (PDB 1VTN), with residues and folds of interest indicated, including α-helix3 (orange), which sits in the major groove of DNA, and Wing2 (cyan), which undergoes frequent mutation in prostate cancer. (b) Kaplan-Meier showing significantly different clinical outcomes of time to biochemical recurrence (BCR, left) or progression to metastatic disease (MET,right) for predicted FOXA1 mutant cases vs. wild-type in the GRID cohort. The difference of MET/BCR survival curves was tested via R survdiff function using G-rho family of tests, without adjustments for multiple comparisons. (c) Associations between predicted FOXA1 mutation status and clinical variables using univariate analysis of the GRID cohort, with FOXA1 wild type as reference. The GRID cohort included 1,626 radical prostatectomy (RP) tumor samples. The center values represent the median odds ratio via univariate analysis. The error bars represent first and third quartiles of odds ratio. The lines represent minimum and maximum odd ratio. Univariate logistic regression analyses were performed on the GRID cohort to test the statistical association between FOXA1 mutant status and clinical variables via generalized linear test, without adjustments for multiple comparisons. The test was two-sided with the significance level of p <0.05 as the cutoff.