Figure 1.

Representative images and directionality analyses for engineered muscle constructs created using flow-alignment method (A-C) compared to conventional mono-dispersion technique (D-F). Fabricated muscle constructs were cultured 24 hours and analyzed by confocal reflection microscopy for visualization of fibril microstructure (A,D), confocal fluorescence microscopy for visualization of myoblast morphology (B,E; green=actin; blue=nuclei), and ImageJ Directionality algorithm for alignment determination (C,F). Scale bar=50μm