Figure 4.

Upregulation of lncRNA FENDRR or Downregulation of miR-423-5p Inhibits the Treg-Mediated Immune Escape of HCC Cells

The MHCC97 cell line was treated with si-lncRNA FENDRR, lncRNA FENDRR, and/or miR-423-5p mimic and co-cultured with Tregs. (A) The FOXP3⁺CD4⁺, FOXP3⁺CD25⁺, and CD8⁺ Tregs examined by flow cytometry after different treatments. (B) The statistical analysis of (A). (C) The levels of IL-10, IFN-γ, TGF-β, VEGF, IL-2, and IL-4 after different treatments detected by ELISA. (D) The immune-suppressive capacity of Tregs after different treatments. *p < 0.05 compared with cells without treatment. The results of positive staining examined by flow cytometry and the results of ELISA were measurement data and expressed as mean ± SD. Data among multiple groups were analyzed by one-way ANOVA, followed by Tukey’s post hoc test. Experiments were repeated three times. HCC, hepatocellular carcinoma; IFN-γ, interferon-γ; IL-4, interleukin-4; IL-10, interleukin-10; lncRNA FENDRR, long non-coding RNA fetal-lethal non-coding developmental regulatory RNA; miR-423-5p, microRNA-423; Treg, regulatory T cell.