Table 1.
Strains and plasmids used in this study.
| Strain and plasmids | Descriptions | Source |
|---|---|---|
| G. japonicus CGMCC 1.49 | Wild-type strain | Preserved in lab |
| E. coli JM109 | Cloning host | Preserved in lab |
| pMD19-T | Cloning vector | Sangon biotech |
| pBBR-MCS | Host for Kana gene | Sangon biotech |
| pMD19-ga5dh-1-sy-Kana-ga5dh-1-xy | Plasmid for gene knocking out | This study |
| pMD19-ga5dh-2-sy-Kana- ga5dh-2-xy | Plasmid for gene knocking out | This study |
| pMD19-ga5dh-1-sy-ga2dh-A-Kana-ga5dh-1-xy | Plasmid for gene knocking out | This study |
| pMD19-ga5dh-1-sy-ga2dh-B-Kana-ga5dh-1-xy | Plasmid for gene knocking out | This study |
| G. japonicus-Δga5dh-1 | Engineered strain | This study |
| G. japonicus-Δga5dh-2 | Engineered strain | This study |
| G. japonicus-Δga5dh-1-ga2dh-A | Engineered strain | This study |
| G. japonicus-Δga5dh-1-ga2dh-B | Engineered strain | This study |