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. 2019 Jul 4;17:551–562. doi: 10.1016/j.omtn.2019.06.019

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© 2019 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

circNrxn2 Increased M2 Macrophage Polarization in HFD Mice

(A) CD206 immunofluorescent staining of HFD mice adipose tissue section. (B) The mRNA levels of IL-6, TNF-α, IL-4, and CD206 in adipose tissue. (C) The mRNA levels of IL-6, TNF-α, IL-4, and CD206 in macrophages. (D) The protein levels of IL-6, TNF-α, IL-10, and CD206 in macrophages. (E) Immunofluorescent staining of CD206. (F) The morphologic observation of macrophages. (G) The mRNA levels of IL-6, TNF-α, IL-4, and CD206 in plasma were detected to evaluate the systemic effects of circNrxn2, miR-103, and FGF10. Data represented the mean ± SEM. Scale bars, 200 μm. *p < 0.05; **p < 0.01. n ≥ 3.