Figure 7.

circNrxn2 Promoted White Adipocyte Browning by Increasing M2 Macrophage Polarization

RAW264.7 macrophages were treated with circNrxn2, circNrxn2 + mi-103, or circNrxn2 + siFGF10 and cultured for 24 h in 2% free fatty acid (FFA)-free BSA in 24-well plates, and the supernatant was collected as RAW-conditioned medium. 3T3-L1 adipocytes were incubated with RAW-conditioned medium for 24 h to detect mRNA expression levels and 48 h for protein levels. (A) The mRNA levels of PRDM16, UCP1, PPARγ, PGC1α, COX7α, and COX8b. (B) The protein levels of PRDM16, UCP1, PPARγ, PGC1α, and COX4. (C) Immunofluorescent staining of UCP1 in adipocytes. Data represent the mean ± SEM. Scale bars, 200 μm. *p < 0.05; **p < 0.01. n ≥ 3.