Figure 1.

Selection of Aptamers that Bind FGFR3

(A) Schematized structure of the aptamers in the library, with an engineered stem loop where the stem allows protrusion of the randomized sequence away from the forward and reverse sequences. (B) Aptamers from the first 6 rounds of selection were incubated with a labeled reverse primer and screened for binding against mouse FGFR3 protein by flow cytometry. (C) Aptamer clones from round 6 were annealed to a labeled reverse primer and screened against mouse and human FGFR3c for binding via flow cytometry; R6c6 on mouse FGFR3c, R6c6 on human FGFR3c, R6c37 on mouse FGFR3c, and R6c37 on human FGFR3c. See the details of selection in Materials and Methods and Table S1.