Figure 2.

Screening Aptamer Clones from Round 6 and Round 10 from Selection and Identifying a Potent Inhibitor, iR3, for FGFR3

(A) Examples of aptamers from round 6 and round 10 of selection competing with 0.2 and 0.4 nM FGF2 on BaF3-R3c:R1c cells. Values are shown as the percentage of growth compared with 0.4 nM FGF2. Round 6 clone 6 (R6c6) robustly inhibited cell growth at a concentration of 1 μmol/L compared with other aptamers from either round 6 or round 10. (B) Full-length parent R6c6 with the reverse primer shown in blue. NK01 is the parent clone without the reverse primer, and the two minimized sequences are NK01.min1 and iR3 with shortened stems. The t denotes an inverted dT placed at the 3′ end during synthesis, whereas s denotes a thiol placed at the 5′ end. (C) Binding of NK01, NK01.min1, and iR3 to mouse and human FGFR3c at a concentration of 100 nmol/L. iR3 retains its ability to bind the target upon minimization from the parent clone.