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. 2019 Jul 4;17:530–539. doi: 10.1016/j.omtn.2019.06.020

Figure 4.

Figure 4

aR3 Stimulates Growth of BaF3-R3c:R1c Cells

(A) Bivalent aptamer transcript sequences constructed to test for agonist activity toward BaF3-R3c:R1c cells. The bivalent aptamers were developed from a modified iR3, the parent molecule of the bivalent aptamers. Different uridine (U) linkers are highlighted in blue, the core randomized sequence in red, and the additional G-C pair in the first monomer in green. (B) Stimulation of BaF3-R3c:R1c cells with the different constructs compared with 0.4 nM FGF2. (C) Calculation of the apparent Kd of aR3 on mouse and human FGFR3c yields a value of ∼189.9 ± 89.1 μmol/L and 710.8 ± 206.5 μmol/L, respectively. All data were acquired from two or more independent experiments.