Fig. 3.

Estrogen signaling effects on the phenotype and function of macrophage infiltrated in inflamed muscle. a FACS analysis of the proportion of Ly-6C⁺F4/80⁺ cells in CD45-gated cells isolated from TA muscle on day 3 post-injury, in OVX or intact mice. b mRNA levels of IL-1β, SLPI, MCP-1, TNF-α, TGF-β2, IL-10, and PPARγ were quantified by qRT-PCR in CD45⁺F4/80⁺ cells isolated from inflamed muscle on day 3 post-injury in OVX or intact female mice. c FACS analysis of the proportion of CD45⁺F4/80⁺MHC-II⁺ cells isolated from inflamed muscle of OVX or intact female mice. d FACS analysis of the proportion of CD45⁺F4/80⁺CX3CR1⁺ cells isolated from inflamed muscle of OVX or intact female mice. e FACS analysis of the uptake of the injected Lumispheres by CD45⁺F4/80⁺ cells isolated from inflamed muscle of OVX or intact female mice. Data are representative of two independent experiments performed with three mice per group. One-way ANOVA was used for multiple comparisons (*p < 0.05)