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. 2019 Jul 29;9:20. doi: 10.1186/s13395-019-0205-2

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Estrogen signaling affects on accumulation and function of Treg cells in damaged muscle. FACS analysis of the frequency of the CD4⁺CD25⁺Foxp3⁺ cells (a), CD4⁺T-bet⁺Foxp3⁺ cells (b), and CD4⁺CD25⁺CTLA-4⁺ cells (c) isolated from 7d-damaged TA muscle in OVX or intact female mice. Data are representative of two independent experiments performed with three mice per group. One-way ANOVA was used for multiple comparisons (*p < 0.05)