Scheme 8. Assembly of de Novo Designed Zinc-Containing Enzymes as Artificial Metallohydrolases.
(a) Zn8·AB34 with structural and catalytic zinc sites highlighted. (b) Hydrolysis of ampicillin by Zn8·AB34 K104A-E57G mutant. (c) Comparison of hydrolysis rates of p-nitrophenol by Zn8·AB34 K104A-E57G and Zn8·AB54. Adapted with permission from ref (35). Copyright 2014 The American Association for the Advancement of Science.