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. 2019 Jul 16;5(7):1120–1136. doi: 10.1021/acscentsci.9b00397

Scheme 9. Design and Directed Evolution of an Artificial Metallohydrolase MID1sc.

Scheme 9

(a) Hydrolysis of profluorescent ester 12 by mutant MID1sc10. (b) Deepening of the hydrophobic binding pocket in MID1sc10 (right) compared to MID1 (left) as a result of directed evolution. (c) Cut-away crystal structure of MID1sc highlighting the tight binding of the enantiopure phosphonate transition state mimic 13. Reproduced with permission from ref (37). Copyright 2018 The American Association for the Advancement of Science.