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. 2019 Jul 25;28(15):1015–1025. doi: 10.1089/scd.2019.0125

FIG. 3.

FIG. 3.

Effects of LPS challenge on the expression of inflammatory response genes, and hPDL cell behavior. (A) Relative IL-1β, DEFA4, and CCL5 mRNA expression in hPDL cells after LPS exposure as compared by RT-qPCR. (B) Western blotting of CCL-5 and IL-1β protein expression in periodontal progenitors treated with PBS control, LPS, or TNF-α. β-Actin was used as an internal control. (C, D) Transwell migration assay demonstrating the migration potential of LPS challenged cells. DAPI staining of hPDL nuclei on fluorescence blocking PET membranes to facilitate quantitation. The relative number of migrated PDL cells was 4.8 (P < 0.05) in the LPS-treated group compared with 1 in the control group. (E, F) Cell migration assessed using the scratch assay. In this assay, PDL cells were subjected to the scratch test. ImageJ was used to demonstrate that after 24 h LPS challenge, there was 30% more wound closure in the LPS treatment group (P < 0.05) compared with the control. PBS, phosphate-buffered saline; PET, polyethylene terephthalate. *p < 0.05; **< 0.01.