. 2019 Jul 25;28(15):1015–1025. doi: 10.1089/scd.2019.0125

Table 1.

Human Chromatin Immunoprecipitation and Reverse Transcriptase-Polymerase Chain reaction Primer Sequences

Oligonucleotides for ChIP (human)
Col1a1	Forward	TTGGGTGTGGCTGTAAGAGA
Col1a1	Reverse	GGGATCTTTCATGGTTTCCA
Col3a1	Forward	TATTTCAGAAAGGGGCTGGA
Col3a1	Reverse	AGTTGTTCCCTGCCCTTCA
Runx2	Forward	AGCAGTTTGCAACCAGACCT
Runx2	Reverse	TGGCTGGATTCCTTCTGTTT
CCL5	Forward	TGAGAGAGCAGTGAGGGAGA
CCL5	Reverse	GCATTGGCCGGTATCATAAG
IL-1β	Forward	AAACCTCTTCGAGGCACAAG
IL-1β	Reverse	GGCAGAGAGACAGAGAGACTCC
DefA4	Forward	TTGGCCTGGATGACCTAGAC
DefA4	Reverse	GCTCCCGAGTAAAGGATGTG
Oligonucleotides for real-time PCR analysis (human)
Col1a1	Forward	CATCTCCCCTTCGTTTTTGA
Col1a1	Reverse	CCAAATCCGATGTTTCTGCT
Col3a1	Forward	GATCAGGCCAGTGGAAATGT
Col3a1	Reverse	GTGTGTTTCGTGCAACCATC
Runx2	Forward	GTGCCTAGGCGCATTTCA
Runx2	Reverse	GCTCTTCTTACTGAGAGTGGAAGG
CCL5	Forward	TGCCCACATCAAGGAGTATTT
CCL5	Reverse	CCAGACTTGCTGTCCCTCTC
IL-1β	Forward	AGTACCTGAGCTCGCCAGTG
IL-1β	Reverse	CTGGAAGGAGCACTTCATCTG
DefA4	Forward	TTCCAGGTCATGGAGGAATC
DefA4	Reverse	CTTTCTTCGGCAGCATTTTC
GAPDH	Forward	ACAGTCAGCCGCATCTTCTT
GAPDH	Reverse	ACGACCAAATCCGTTGACTC
β-actin	Forward	GATGAGATTGGCATGGCTT
β-actin	Reverse	CACCTTCACCGTTCCAGTTT

ChIP, chromatin immunoprecipitation; PCR, polymerase chain reaction.