. 2019 Jul 25;28(15):1004–1014. doi: 10.1089/scd.2019.0124

Table 1.

Oligonucleotides for ChIP Analysis and Real-Time Expression Analysis

Oligos for ChIP
hCTNNB1	Forward	TGGAACCAGATAAAAATGGAATC
hCTNNB1	Reverse	AGCCCGCAATTCAACAAGT
hSFRP1-400	Forward	GCTTAACCCTCCCTCTCAGG
hSFRP1-400	Reverse	GACGGTATCAGCACAACAGC
hSFRP1-1300	Forward	GCAGTCAGCGGAGATAGC
hSFRP1-1300	Reverse	GGGGAGCCTGGATCATACTT
hRUNX2-3000	Forward	GTGGGACTCGAGAGACCTGT
hRUNX2-3000	Reverse	AGACCGGACCAACTGGAAG
hRUNX2-2000	Forward	GCGTTTGCACTGAGCAATAA
hRUNX2-2000	Reverse	CCTCGAAGCATCAAGGAAGA
hRUNX2-1900	Forward	CGCAAACACGTTTTCAAGC
hRUNX2-1900	Reverse	GGAAATCCGCTTGAGGCTAT
hSP7-3500	Forward	TCCCCATCTTTGTTCCTCTG
hSP7-3500	Reverse	GAAGACCAGATGTGTGGCTTTA
hSP7-3100	Forward	TCTATCAGCCACCTGGTTCC
hSP7-3100	Reverse	CAGCAGGTAGGCACCAATTT
hRUNX2 bCATbind	Forward	CGTAGTAGTACACAACGCCG
hRUNX2 bCATbind	Reverse	GTTTCGTGTCTGTCTTCCCC
Oligos for real-time RT-PCR analysis
hBMP2	Forward	TCAAGCCAAACACAAACAGC
hBMP2	Reverse	AGCCACAATCCAGTCATTCC
hBMP4	Forward	CTTTACCGGCTTCAGTCTGG
hBMP4	Reverse	ATGTTCTTCGTGGTGGAAGC
hCTNNB1	Forward	TCTGAGGACAAGCCACAAGATTACA
hCTNNB1	Reverse	TGGGCACCAATATCAAGTCCAA
hMSX2	Forward	CGGAAAATTCAGAAGATGGAG
hMSX2	Reverse	GAGGAGCTGGGATGTGGTAA
hRUNX2	Forward	GTGCCTAGGCGCATTTCA
hRUNX2	Reverse	GCTCTTCTTACTGAGAGTGGAAGG
hSP7	Forward	TACCCCATCTCCCTTGACTG
hSP7	Reverse	GCAACAGGGGATTAACCTGA
hIBSP	Forward	AACCTACAACCCCACCACAA
hIBSP	Reverse	CGTACTCCCCCTCGTATTCA
hBGLAP	Forward	GACTGTGACGAGTTGGCTGA
hBGLAP	Reverse	AGCAGAGCGACACCCTAGAC
hCOL I	Forward	GGAGCTCCAAGGACAAGAAA
hCOL I	Reverse	ATGAAGGCAAGTTGGGTAGC
hALP	Forward	CAACCCTGGGGAGGAGAC
hALP	Reverse	GCATTGGTGTTGTACGTCTTG
hGAPDH	Forward	ACAGTCAGCCGCATCTTCTT
hGAPDH	Reverse	ACGACCAAATCCGTTGACTC

ChIP, chromatin immunoprecipitation; RT-PCR, real-time polymerase chain reaction.