miR-200c reduces protein level of proinflammatory and osteoclastogenic mediators after LPS stimulation. (A–E) Relative fold change of transcripts of IL-6
(A), IL-8
(B), p50
(C), p65
(D), and IFRD1
(E) in HGFs with different transfections 24 h after stimulation with 1 μg/mL of LPS-PG. *P < 0.05 versus HGFs untreated with LPS; #P < 0.05 versus LPS-EV; performed in replicate. (F)
Targetscan.org predicted the possible binding site of miR-200c-p3 and the 3′UTR of IFRD1 at the position 1,120–1,126. IFRD1, interferon-related developmental regulator-1; LPS-PG, Porphyromonas gingivalis lipopolysaccharide.