Table 1.
Sequence for Forward and Reverse Primer Sets Used for Real-Time Polymerase Chain Reaction
| Primer | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
| Human primers | ||
| IL-6 | CCATCTTTGGAAGGTTCAGGTTG | ACTCACCTCTTCAGA ACGAATTG |
| IL-8 | AACCCTCTGCACCCAGTTTTC | ACTGAGGATTGAGAGTGGAC |
| CCL-5 | TGCCCACATCAAGGAGTATTT | CTTGCTGTCCCTCTCTCTTTG |
| IFRD1 | TGCAGTGGTTATAGCGATCCT | CCTTGTCTTCGCACTCTTATCC |
| P65 | CGCTGCTCTAGAGAACACAATGGCCACTTGCCG | GCGGCCAAGCTTAAGATCTGCCGAGATAAC |
| p50 | ATGTATGTGAAGGCCCATCC | TTGCTGGTCCCACATAGTTG |
| VPS29 | GAATAACTTGGTGTCCGTGGAT | GTGAGAGGAGACTTCGATGAGA |
| Rat primers | ||
| IL-6 | GCCCTTCAGGAACAGCTATGA | TGTCAACAACATCAGTCCCAAGA |
| IL-8 | CATTAATATTTAACGATGTGGATGCGTTTCA | GCCTACCATCTTTAAACTGCACAAT |
| IFRD1 | ATCGGACTGTTCAACCTTTCAG | GCACTCTTATCAAGGGTTAGGTC |
| RUNX2 | CTTCAAGGTTGTAGCCCTCG | TAGTTCT CATCATTCCCGGC |
| ALP | GCAGGATCGGAACGTCAAT | ATGAGTTGGTAAGGCAGGGTC |
| RANKL | CCCATCGGGTTCCCATAAAGTC | GCCTGAAGCAAATGTTGGCGTA |
| OPG | GTCCCTTGCCCTGACTACTCT | GACATCTTTTGCAAACCGTGT |
| RPS18 | GTGATCCCCGAGAAGTTTCA | AATGGCAGTGATAGCGAAGG |
ALP, alkaline phosphatase; CCL, chemokine (C-C motif) ligand; IFRD1, interferon-related developmental regulator-1; IL, interleukin; OPG, osteoprotegerin; RANKL, receptor activator of nuclear factor kappa-B ligand; RPS18, ribosomal protein gene; RUNX2, runt related transcription factor 2; VPS29, vacuolar protein sorting 29 homolog.