Figure 3.

Purified Sl CYS8 suppresses labelling of PLCPs in total leaf extracts and apoplastic fluids. (a) Total extracts (TE) and (c) apoplastic fluids (AF) from plants agroinfiltrated with an empty vector control were isolated and pre‐incubated with various concentrations of Sl CYS8 purified from E. coli, prior to PLCP labelling with MV201. Quantification of fluorescent band intensity (b and d) shows that PLCP activity is dependent on Sl CYS8 concentration. As a control, plant extracts were mixed and pre‐incubated with E‐64 inhibitor before MV201 labelling. A Coomassie blue‐stained gel shows Sl CYS8 in the samples.