Skip to main content
. 2019 Jul 3;8(14):e012508. doi: 10.1161/JAHA.119.012508

Figure 2.

Figure 2

Comparison of wild‐type (WT) and mutant ARMC5 (rs116201073) expression in human embryonic kidney 293 (HEK293) cell line. A and B, Real‐time quantitative polymerase chain reaction (RTqPCR) using primers recognizing all ARMC5 isoforms (A) or only the ARMC5‐203 isoform (B) on HEK293 cells transfected with WT or mutant (variant‐carrying) ARMC5‐203 plasmid for 24 and 48 hours. C, The ratio of the mutant and the WT ARMC5‐203 expression detected by RTqPCR on HEK293 cells untreated or treated 1, 2, or 3 hours with a translation blocker, cycloheximide. The graph represents the means of at least 3 independent experiments±standard error of the mean (SEM).