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. 2019 Jul 15;8(14):e012486. doi: 10.1161/JAHA.119.012486

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© 2019 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Alizarin red staining for calcium. A, Left anterior descending artery (LAD, top panels), proximal segment of the septal artery (middle panels), and the aortic root (bottom panels). B through D, Morphometric quantification of alizarin red positive area in the LAD (B), septal artery (C), and the aortic root (D). *P<0.05, ***P<0.001. eTNAP indicates overexpression of tissue‐nonspecific alkaline phosphatase in the endothelium; hArg, homoarginine; LAD, left anterior descending artery; WHC, wicked high‐cholesterol allele.